The Structure Of The T2 Onc2 Transposon Used In Sb Screening A The

The Structure Of The T2 Onc2 Transposon Used In Sb Screening A The The first landmark paper used t2 onc2 transposon transgenic mice and conditional sb11 knock‐in mice to induce mutagenesis in intestinal epithelial cells. the triple compound mice carrying t2 onc2, sb11, and villin‐cre survived longer than 1.5 y and developed intestinal tumors with long latency. analysis of 135 tumors identified 77 ccgs genes. The structure of the t2 onc2 transposon used in sb screening. a, the t2 onc2 transposon has unique inverted repeat sequences (irdrl and irdrr) at each end that are recognized by the sb transposase.

The Structure Of The T2 Onc2 Transposon Used In Sb Screening A The Single gene transgenic rats can be interbred to obtain double transgenic rats. 64 with the help of electroporation method, sb transposon system can also deliver c myc, h ras, and shp53 oncogenes into rats to produce liver tumor. 65 in addition, the sb100x transposon system has been ever used for enzyme catalyzed gene integration into the. Changes to the transposon structure further changed the severity of disease as well as the tumor spectrum observed. t2 onc2 is a smaller transposon, with optimised transposase binding sites and a larger fragment of the engrailed 2 splice acceptor [2]. three t2 onc2 lines with an estimated 30–77 unmethylated copies were established [2]. The t2 onc2 transposon was made by replacing the hpai bglii fragment containing the en2 splice acceptor from pt2 onc with a fragment containing a larger portion of the en2 exon. in addition to. We generated a new mutagenic transposon, t2 onc3, the structure of which is identical to that of t2 onc2 except that the mscv ltr is replaced with the cag promoter . this promoter consists of the cytomegalovirus immediate early enhancer fused to the cag promoter, and expresses strongly in a variety of cell types in vivo , including epithelial.